Laboratory testing of the device Ecofood's impact

Staph cells (Staphylococcus) taken in advance from a suppurating wound of a patient and multiplied in the growth medium represented researching biomaterial. This material was studied in order to determine its high resistance to antiseptics, heat treatment, comparatively high survival and danger of this bacterium for health of people and animals.

The following equipment was utilized during the study: a standard laboratory microscope and vital colorant (that doesn't damage vital processes of bacteria). Weak electromagnetic field of variable polarity was utilized in order to define amplitude of mobility, determining by superficial electric charge of bacteria. As it is known, unlived bacteria have absolute zero superficial charge; and they are absolutely unmovable in electromagnetic field. All viable bacteria, without any exception, are movable in electromagnetic field. Such mobility is provided by superficial electric charge composed because of internal metabolism and ferment chemical reactions inside of bacteria's cell. Colorant, 0.1%, Methylene Blue, vital.

Research #1: Standard laboratory microscope. Enhancement x900, inserted digital video recorder. The research was conducted with the help of alternating electromagnetic field created artificially. Impact was exercised by the device Ecofood once. Standard exposure lasted 20 minutes. The distance between the preparation and the device was about 15 cm.

Planning of experimental projects. Cernevtseva Larisa V., doctor of medical sciences, Soloviov Alexander A., Head of Cytology's Department:

Preparation for the research. Setup of equipment:

The first experiments:

Attempts to improve quality of pictures' visualization:

Staph cells without impact of Ecofood upon them:

Staph cells after impact of Ecofood upon them:

The 15^th minute of the device Ecofood's impact. A. A. Soloviov comments the fact of painting Staph cells because of the device's impact on them:

Staph bacterium and photo of wounded surfaces with infected sources:

Results of the research and conclusions:

Staphylococcus is completely (100%) painted into the color of vital colorant in 15 minutes of the device's impact upon it. It may indicate about partial or total coagulation of protein in membrane shell of Staphylococcus, it means that micro cracks and pores appear in it. It has been observed that the colorant is passing into cells of the Staphylococcus step-by-step during 10-15 minutes. You can clearly see it, if you compare the image related to the state before impact of the device and after it (both video clips are above). It doesn't happen without impact of the device Ecofood. It is necessary to mention here about the Soviet Union's scientist Dmitriy M. Nosonov's theory supposed in 1925 and published in his work "Reaction of viable substance towards external impacts" in 1941. According to the theory, microorganism's painting into the color of a colorant is an absolute signal which indicates that any microorganism has begun to die because the reason of the painting is damage of its cellular membrane's integrity. D. M. Nosonov has acquired Stalin's prize of the 2^nd level (1943) and worldwide recognition of his works. His works are still universally recognized and uncontested.
It is observed unessential but obvious reduction of amplitude of cells' movement in electromagnetic field during last moments of the device's impacts upon Staph cells. It means that superficial electric charge of cells reduces, in other words: internal ferment reactions insides of Staph cells slow down. It becomes obvious, if you compare two video clips. Alternating electromagnetic field was created by the device "Bio-Test Axion"; the intensity of the field before, during and after the impact wasn't changed. Electromagnetic field was needed just for management of cell's vitality; it was also turned out in each 3-5 minutes for observation of the ongoing process.

The experiment was conducted 2-3 times daily during 4 days. Impact of the device was single each time (20 minutes). Unfortunately, more prolonged researches about the process of Staph cells' vitality couldn't be conducted in this laboratory because of rapid drying (40 minutes) of fresh prepared composition. The problem was linked only with way of preparing biomaterial. The task for further researches is following: it is necessary to do the same actions but it is needed to use other ways of preparing material.

Research #2: The research was conducted in the Department of Dentistry (Izhevsk Medical Institute). Dark field microscope (enhancement x500; inserted video recorder) was used during the experiment. Prepared saliva served as biological material. It allowed increasing duration of the device Ecofood's impact by three times (20 minutes per each time) with intervals of 5-10 minutes between exposures. The distance between the preparation and the device was about 20 cm. It was not required to create artificial alternating electromagnetic field. This biomaterial usually has very mobile microflora. Preparations made of saliva were quite successful: there were no cases of rapid drying or penetration of air bubbles between the glasses, as it was in the case of the preparations which contained Staph infections.

4 video clips are presented below. The 1st video clip: the preparation before impact of the device Ecofood upon it; the 2nd - 4th video clips: the 1st, the 2nd and the 3rd impact of the device upon the preparation. Screenings took place after each impact. Blue vital colorant was used during the research. Big blue formations presented epithelium cells of skin and mucosa. Small moveable objects represented microorganisms. It was impossible to identify data of the organism during such enhancement; but the research didn't have this task at that stage.

Prepared saliva before impact of the device Ecofood. Numerous moveable microorganisms are observed:

1^st impact of the device:

2^st impact of the device:

3^st impact of the device. Almost all microorganisms are unmovable, or non-viable. Just one slow-moving microorganism is observed on some shots (1min 42sec):

Results and conclusions: Effectiveness of the device Ecofood's work is clearly and certainly confirmed during the experiment. Almost the whole microflora of the prepared saliva has become non-viable after the 3^rd impact.

General conclusions:
The following conclusions have been made on the basis of these two various experiments conducted with the help various biomaterials, different methods for determination of bacteria's viability and different optics:

The device Ecofood influences on viability of microorganisms obviously and certainly. Viability of organisms exposed to the impact of the device Ecofood reduces. Membrane's protein of Staph cells coagulates and gives micro cracks in 15 minutes of exposure; it certainly reduces and neutralizes dangerous properties of these cells. The process of complete destruction of Staph cells is an aspect for further research in this direction, if it is needed. Staph infections are claimed to be bacteria, having high viability and adaptation to harsh conditions of survival.

Bacteria observed in the prepared saliva die completely after the 3rd impact of the device Ecofood. It was impossible to influence on the saliva once and then observe viability of bacteria in saliva each hour with the help of the administered equipment because fresh prepared saliva may be studied only during an hour and a half; then the preparation dies which may affect viability of bacteria naturally. That's why it didn't make any sense to continue studying the same preparation over an hour and a half.
Impact of the device Ecofood and viability of exposed microorganisms are determined in accordance with the following principle: the more often impact is exercised, the higher level of sterilization is observed.