Staph cells (Staphylococcus) taken in advance from a suppurating wound of a patient and multiplied in the growth medium represented researching biomaterial. This material was studied in order to determine its high resistance to antiseptics, heat treatment, comparatively high survival and danger of this bacterium for health of people and animals.
The following equipment was utilized during the study: a standard laboratory microscope and vital colorant (that doesn't damage vital processes of bacteria). Weak electromagnetic field of variable polarity was utilized in order to define amplitude of mobility, determining by superficial electric charge of bacteria. As it is known, unlived bacteria have absolute zero superficial charge; and they are absolutely unmovable in electromagnetic field. All viable bacteria, without any exception, are movable in electromagnetic field. Such mobility is provided by superficial electric charge composed because of internal metabolism and ferment chemical reactions inside of bacteria's cell. Colorant, 0.1%, Methylene Blue, vital.
Research #1: Standard laboratory microscope. Enhancement x900, inserted digital video recorder. The research was conducted with the help of alternating electromagnetic field created artificially. Impact was exercised by the device Ecofood once. Standard exposure lasted 20 minutes. The distance between the preparation and the device was about 15 cm.
Planning of experimental projects. Cernevtseva Larisa V., doctor of medical sciences, Soloviov Alexander A., Head of Cytology's Department:
Preparation for the research. Setup of equipment:
The first experiments:
Attempts to improve quality of pictures' visualization:
Staph cells without impact of Ecofood upon them:
Staph cells after impact of Ecofood upon them:
The 15th minute of the device Ecofood's impact. A. A. Soloviov comments the fact of painting Staph cells because of the device's impact on them:
Staph bacterium and photo of wounded surfaces with infected sources:
Results of the research and conclusions:
Research #2: The research was conducted in the Department of Dentistry (Izhevsk Medical Institute). Dark field microscope (enhancement x500; inserted video recorder) was used during the experiment. Prepared saliva served as biological material. It allowed increasing duration of the device Ecofood's impact by three times (20 minutes per each time) with intervals of 5-10 minutes between exposures. The distance between the preparation and the device was about 20 cm. It was not required to create artificial alternating electromagnetic field. This biomaterial usually has very mobile microflora. Preparations made of saliva were quite successful: there were no cases of rapid drying or penetration of air bubbles between the glasses, as it was in the case of the preparations which contained Staph infections.
4 video clips are presented below. The 1st video clip: the preparation before impact of the device Ecofood upon it; the 2nd - 4th video clips: the 1st, the 2nd and the 3rd impact of the device upon the preparation. Screenings took place after each impact. Blue vital colorant was used during the research. Big blue formations presented epithelium cells of skin and mucosa. Small moveable objects represented microorganisms. It was impossible to identify data of the organism during such enhancement; but the research didn't have this task at that stage.
Prepared saliva before impact of the device Ecofood. Numerous moveable microorganisms are observed:
1st impact of the device:
2st impact of the device:
3st impact of the device. Almost all microorganisms are unmovable, or non-viable. Just one slow-moving microorganism is observed on some shots (1min 42sec):
Results and conclusions: Effectiveness of the device Ecofood's work is clearly and certainly confirmed during the experiment. Almost the whole microflora of the prepared saliva has become non-viable after the 3rd impact.
The following conclusions have been made on the basis of these two various experiments conducted with the help various biomaterials, different methods for determination of bacteria's viability and different optics: